Comparative analysis of active networks reveals the changes of key proteins and their interactions under different oxygen levels in Shewanella oneidensis MR-1

Abstract Purpose The extracellular electron transfer (EET) capability enables electroactive microorganisms have a wide range of applications in energy production, wastewater treatment, bioremediation and chemical synthesis. Because the oxygen levels will sharply affect EET process, we integrated transcriptome changes under different with protein–protein interaction (PPI) network to study specific these conditions typical microorganism Shewanella oneidensis MR-1 ( S. MR-1). Methods First, mRNA expression data were into its PPI construct active protein networks. Then, studied key proteins their interactions by comparative analysis multiple pairs Results node centrality networks high low shows that most nodes are ribosomes or closely related ribosomes. results our versus change show two FlgB PetA largest changed proteins. Among proteins, is structural component flagella, while cytochrome c . Furthermore, also analyzed levels, identified each pair networks, finally screened out Tig-RplX exists three Conclusion Our indicate translational processes corresponding translation efficiency may play an important role before after activation process MR-1. this can provide some guidance for identifying species.